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Journal
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Immunology and Infection
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人类舱内单聚物研究病原体、共脉和宿主肠道上皮细胞之间的相互作用
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium
Please note that all translations are automatically generated.
Click here for the English version.
人类舱内单聚物研究病原体、共脉和宿主肠道上皮细胞之间的相互作用
DOI:
10.3791/59357-v
•
07:20 min
•
April 09, 2019
•
Julie G. In*
1
,
Jennifer Foulke-Abel*
2
,
Elizabeth Clarke
,
Olga Kovbasnjuk
1
Department of Internal Medicine
,
University of New Mexico Health Science Center
,
2
Department of Medicine, Division of Gastroenterology & Hepatology
,
Johns Hopkins University School of Medicine
Chapters
00:04
Title
01:22
Isolation and Dissociation of Enteroids/Colonoids from 3D Culture
02:39
Plating the Enteroid/Colonoid Suspension
04:10
Fixation to Preserve and Immunostain Mucus
05:16
Results: Establishment and Evaluation of Human Enteroid/Colonoid Monolayers
06:51
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个培养人类肠体或类团类单层具有完整的屏障功能的方案, 以研究宿主上皮微生物在细胞和生化水平上的相互作用。
Tags
Colonoid Monolayers
Intestinal Epithelium
Host-pathogen Interaction
Mucus Biology
Microbiome
Inflammatory Bowel Disease
3D Organoid Culture
Ex Vivo Studies
Harvesting Solution
Cell Suspension
DMEM/F12 Medium
Cell Plating
Expansion Medium
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