JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
冷冻斑马鱼胚胎的连续免疫荧光与免疫组化学
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos
Please note that all translations are automatically generated.
Click here for the English version.
冷冻斑马鱼胚胎的连续免疫荧光与免疫组化学
DOI:
10.3791/59344-v
•
09:20 min
•
May 14, 2019
•
Jordan L. Ferguson*
,
Heather R. Shive*
1
Department of Population Health and Pathobiology
,
NC State University College of Veterinary Medicine
Chapters
00:04
Title
01:06
Embryo Embedding and Preparation of Cryosections
02:31
Immunofluorescence for pH3
04:54
Immunohistochemistry for Labeled Dextran
07:50
Results: Identification of Individual Cells After Immunofluorescence and Immunohistochemistry
08:21
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该方案演示了早期斑马鱼胚胎的冷冻节的连续免疫荧光和免疫组织化学,从而对特定细胞群进行精确的共定位分析。
Tags
Immunofluorescence
Immunohistochemistry
Cryosections
Zebrafish Embryos
Co-localization
Antibody Compatibility
Cryosectioning
Co-expression
Embryo Orientation
Cryostat
Charged Glass Slides
PBS Wash
Humid Chamber
Barrier Pen
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
使用荧光抗体对小鼠表皮石蜡切片的免疫组织化学
显微注射mRNA和斑马鱼的胚胎吗啉反义寡核苷酸。
斑马鱼的胚胎显微注射来分析基因的功能
双光子现场斑马鱼的胚胎干切断和时间推移共焦成像
移植嵌合斑马鱼胚胎生成
在现场的斑马鱼胚胎的基于双光子Photoactivation
腹腔注射到成年斑马鱼
在斑马鱼胚胎的代谢标记和Bioorthogonal点击化学成像聚糖
免电力,顺序的核酸和蛋白质的分离
斑马鱼胚胎的代谢谱分析
Read Article