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Journal
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Immunology and Infection
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用荧光显微镜分析膜受体扩散和簇簇的图像处理协议
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
用荧光显微镜分析膜受体扩散和簇簇的图像处理协议
DOI:
10.3791/59314-v
•
12:15 min
•
April 09, 2019
•
Carlos Oscar S. Sorzano*
1,2
,
Laura Martínez-Muñoz*
3,4
,
Graciela Cascio
5
,
Eva M. García-Cuesta
,
J. Vargas
6
,
Mario Mellado
,
Jose Miguel Rodriguez Frade
1
Department of Macromolecular Structures, Centro Nacional de Biotecnología
,
Campus Univ. Autónoma de Madrid
,
2
Campus Urb. Montepríncipe s/n
,
Univ. San Pablo CEU
,
3
Department of Immunology and Oncology, Centro Nacional de Biotecnología
,
Campus Univ. Autónoma de Madrid
,
4
Department of Cell Signaling
,
Centro Andaluz de Biología Molecular y Medicina Regenerativa (CSIC)
,
5
Meyer Cancer Center
,
6
Department of Anatomy and Cell Biology
,
McGill Univ.
Chapters
00:04
Title
01:37
Preparation of Biological Samples and Image Acquisition
02:57
Selection of Images and Creation of Masks
05:45
Tracking Particles
08:09
Calculation of the Diffusion Coefficients and Classification of Trajectories
09:10
Calculation of Cluster Size
09:50
Results: Analysis of Dynamic Fluorescence Particle Characteristics
11:24
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个单一粒子跟踪图像分析的协议, 允许定量评估扩散系数, 类型的运动和集群大小的单个粒子检测到的荧光显微镜。
Tags
Image Processing
Single-molecule Tracking
Lateral Diffusion
Spot Size
Membrane Receptors
Fluorescence Microscopy
TIRF Microscopy
Jurkat Cells
Chemokine Receptor
CXCL12
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