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脑肿瘤细胞行为的纵向生命成像,以响应侵入性手术活检
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
Longitudinal Intravital Imaging of Brain Tumor Cell Behavior in Response to an Invasive Surgical Biopsy
DOI:

09:17 min

May 03, 2019

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Chapters

  • 00:04Title
  • 00:37Surgical Preparation
  • 02:16Tumor Cell Injection and Cranial Imaging Window (CIW) Preparation
  • 03:26Intravital Imaging Setup
  • 04:07Time-Lapse Image Acquisition
  • 05:02Biopsy-Like Injury and CIW Replacement
  • 05:50Image Analysis
  • 06:56Results: Representative Impact of Biopsy on Tumor Cell Migration
  • 08:56Conclusion

Summary

Automatic Translation

在这里,我们描述了一种在同一活的动物内进行侵入性手术(例如活检)之前和之后对脑肿瘤细胞进行高分辨率延时多光子成像的方法。这种方法允许研究这些侵入性外科手术对肿瘤细胞在单个细胞水平的迁移、侵入性和增殖行为的影响。

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