JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Cancer Research
/
キメラ抗原受容体の T 細胞の抗腫瘍機能の予測評価のため培養腫瘍細胞の再投与に
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal
Cancer Research
In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function
Please note that all translations are automatically generated.
Click here for the English version.
キメラ抗原受容体の T 細胞の抗腫瘍機能の予測評価のため培養腫瘍細胞の再投与に
DOI:
10.3791/59275-v
•
08:04 min
•
February 27, 2019
•
Dongrui Wang
2
,
Renate Starr
,
Darya Alizadeh
,
Xin Yang
,
Stephen J. Forman*
1
,
Christine E. Brown*
1
1
Department of Hematology & Hematopoietic Cell Transplantation, T Cell Therapeutics Research Laboratory
,
City of Hope Beckman Research Institute and Medical Center
,
2
Irell and Manella Graduate School of Biological Sciences
,
City of Hope Beckman Research Institute and Medical Center
Chapters
00:04
Title
00:54
Tumor Cell: T Cell Co-culture Setup and Tumor Cell Rechallenge
02:37
Sample Harvest for Flow Cytometric Analysis
04:12
Functional and Phenotypic Chimeric Antigen Receptor (CAR) T Cell Analysis
05:14
Results: Representative CD4+ and CD8+ CAR T Cell Phenotypes After Repetitive Challenge
07:30
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
ここでは、抗腫瘍 T 細胞の活動の表現型および機能解析を可能にする T 細胞を腫瘍をターゲットとした再帰的に挑戦、体外培養法について述べる。
Tags
In Vitro
Tumor Cell
Rechallenge
Chimeric Antigen Receptor
CAR T Cells
Anti-tumor Function
Glioblastoma
Tumorspheres
Co-culture
High Throughput Screening
Cell Dissociation
Cell Suspension
Cell Culture
Supernatant
Trypsin EDTA
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
単一のセル下流解析のターゲット細胞前濃縮と全体のゲノム増幅
同系マウス B 細胞リンパ腫モデル CD19 車 T 細胞の前臨床評価
CD8 による培養腫瘍細胞のアポトーシスのリアルタイム検出
+
T 細胞腫瘍浸潤骨髄細胞の免疫抑制機能を勉強するには
マウス造血幹細胞および前駆細胞およびMLL-AF9駆動白血病におけるミトコンドリア活性酸素種のフローサイトメトリック解析
キメラ抗原受容体(CAR)T細胞の養子免疫療法用
腫瘍抗原特異的T細胞の生成にヒト誘導多能性幹細胞を用いた
直交トピック膵腫瘍の生成と腫瘍浸潤T細胞細胞傷害の
Ex生体内
特性評価
ヒト乳腺上皮細胞における発癌性転換のインビトロ評価
[
18F
]テトラフルオロホウ酸陽電子放射断層撮影/コンピュータ断層撮影によるキメラ抗原受容体T細胞の動的イメージング
ポリ無水物IL-1αナノ粒子の
生体内
抗腫瘍活性の評価
Read Article