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Neuroscience
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膜靶向 ca 2 + 指标对培养细胞中局部 Ca
2 +
信号的解剖
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Dissection of Local Ca
2+
Signals in Cultured Cells by Membrane-targeted Ca
2+
Indicators
Please note that all translations are automatically generated.
Click here for the English version.
膜靶向 ca 2 + 指标对培养细胞中局部 Ca
2 +
信号的解剖
DOI:
10.3791/59246-v
•
11:33 min
•
March 22, 2019
•
Hiroko Bannai
2
,
Matsumi Hirose
,
Fumihiro Niwa
3
,
Katsuhiko Mikoshiba
1
Japan Science and Technology Agency
,
PRESTO
,
2
Laboratory for Developmental Neurobiology
,
RIKEN Center for Brain Science
,
3
École Normale Supèrieure, Institut de Biologie de l’ENS (IBENS), Institut national de la santè et de la recherche mèdicale (INSERM), Centre national de la recherche scientifique (CNRS), École Normale Supèrieure, PSL Research University
Chapters
00:04
Title
01:10
Preparation of Poly(ethyleneimine) Coated Coverslips
02:42
Preparation of Rat Cortical Neuron-astrocyte Mixed Culture and Frozen Cells, and the Revival of Frozen Cultures
05:45
Transfection and Adeno-associated Virus Infection of Hippocampal or Cortical Neurons
07:29
Recording Spontaneous Activities of Astrocytes Expressing Lck-RCaMP2 and OER-GCaMP6f
08:37
Results: Dissection of Local Ca
2+
Signals in Cultured Cells
10:38
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个协议 ca
2 +
成像在神经元和胶质细胞, 使 ca
2 +
信号的解剖在亚细胞分辨率。此过程适用于允许表达基因编码的 ca
2 +
指标的所有细胞类型。
Tags
Ca2+ Signals
Calcium Imaging
Genetically Encoded Calcium Indicators
Subcellular Resolution
Calcium Influx
Calcium Release
Cell Culture
PEI Coating
Cell Dissociation
Cortex
Trypsin
DNase
Plating Medium
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