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一种新的坏死性肠结肠炎人上皮肠素模型

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Immunology and Infection

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JoVE Journal Immunology and Infection

A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis

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一种新的坏死性肠结肠炎人上皮肠素模型

DOI:

10.3791/59194-v

•

08:42 min

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April 10, 2019

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Guillermo J. Ares, Christie Buonpane, Carrie Yuan, Douglas Wood, Catherine J. Hunter²

¹Department of Pediatrics,Northwestern University, ²Division of Pediatric Surgery,Ann & Robert H. Lurie Children’s Hospital of Chicago

Chapters

00:04Title
01:25Crypt Isolation and Plating from Whole Tissue
05:21Induction of Experimental NEC and Preparation for Paraffin Embedding
07:22Results: Analysis of Enteroids
08:21Conclusion

Summary

Automatic Translation

肠类正在成为人类疾病研究的新模式。该协议描述了如何模拟人坏死性小肠结肠炎的肠素模型使用脂多糖 (LPS) 治疗从新生儿组织产生的肠内化合物。收集的肠素显示炎症变化类似于那些在人类坏死性小肠结肠炎。

Tags

Human Epithelial Enteroids Necrotizing Enterocolitis Intestinal Stem Cells Organoids Cell Isolation Inflammation Intestinal Epithelium Co-administration Lipopolysaccharide

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