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Journal
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Biology
/
結合されたヌクレオチドおよび
線虫
のタンパク質抽出
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Combined Nucleotide and Protein Extractions in
Caenorhabditis elegans
Please note that all translations are automatically generated.
Click here for the English version.
結合されたヌクレオチドおよび
線虫
のタンパク質抽出
DOI:
10.3791/59178-v
•
10:37 min
•
March 17, 2019
•
Joslyn Mills
,
Erin McConnell
,
Joshua A. Leitão
,
Louis R. Lapierre
1
Department of Molecular Biology, Cell Biology and Biochemistry
,
Brown University
Chapters
00:04
Title
00:33
Sample Collection
01:31
Nucleotide and Protein Isolation
07:01
Evaluating mRNA with RT-qPCR
08:12
Results: Extractions of Different Macromolecules from the Same
C. elegans
Sample
09:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
変動が減少し、再現性を向上させるための解釈を容易にするための努力で同じサンプルからの蛋白質、DNA、RNA の隔離のためのプロトコルを紹介します。
Tags
Caenorhabditis Elegans
Nucleotide Extraction
Protein Extraction
MRNA
Macromolecules
Sample Variability
Reproducibility
Sample Collection
Cross-sectional Analysis
Worms
M9 Buffer
GTCP Reagent
Chloroform
RNA Isolation
Isopropanol
Ethanol
DNA Isolation
Protein Isolation
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