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Immunology and Infection

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巨噬细胞 ros 产生的流式细胞仪测量对 fcγr 交联的响应

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Immunology and Infection

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JoVE Journal Immunology and Infection

Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking

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巨噬细胞 ros 产生的流式细胞仪测量对 fcγr 交联的响应

DOI:

10.3791/59167-v

•

13:20 min

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March 07, 2019

•

Michael G. Shehat, Justine Tigno-Aranjuez

¹Division of Immunity and Pathogenesis, Burnett School of Biomedical Sciences,University of Central Florida

Chapters

00:04Title
01:22Reagent and Cell Preparation for ROS Measurement
05:02Assay Preparation
05:53Performing the Assay
11:28Results: Flow Cytometric Measurement of ROS Production in Macrophages in Response to FcγR Cross-linking
13:00Conclusion

Summary

Automatic Translation

这项研究表明, 使用流式细胞仪检测活性氧 (ros) 的产生, 由于激活的 fcγr。该方法可用于评估吞噬细胞对免疫复合物、光化微生物或直接 fcγr 交联的抗菌和氧化还原信号功能的变化。

Tags

Flow Cytometry ROS Production Macrophages Fc-gamma Receptor Crosslinking Oxidative Stress Superoxide Chronic Granulomatous Disease Autoimmune Diseases Neurodegenerative Diseases

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