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髓鞘有机小脑片培养的制备及免疫染色
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures
Please note that all translations are automatically generated.
Click here for the English version.
髓鞘有机小脑片培养的制备及免疫染色
DOI:
10.3791/59163-v
•
09:41 min
•
March 20, 2019
•
Melina Thetiot
,
Rémi Ronzano
,
Marie-Stéphane Aigrot
,
Catherine Lubetzki
,
Anne Desmazières
1
Sorbonne Université, Inserm, CNRS, Institut du Cerveau et de la Moelle épinière, ICM-GH Pitié-Salpétrière
Chapters
00:04
Title
00:36
Cerebellum Dissection and Sections Preparation
03:58
Slices Culture and Demyelination
04:55
Immunohistochemistry
08:15
Results: Myelin Immunostaining in Organotypic Cerebellar Slices
09:03
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文提出了一种利用免疫组织化学方法制备小鼠小脑和髓鞘染色的有机切片培养方法, 该方法适用于研究中枢神经系统髓鞘和再髓鞘的机制。
Tags
Myelinating Organotypic Cerebellar Slice Cultures
Myelination
Remyelination
Cerebellum
Dissection
Immunostaining
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