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Cryo-scanning Electron mikroskop ile görselleştirilen Woody bitkilerde xylem su dağılımı
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1Department of Plant Ecology,Forestry and Forest Products Research Institute (FFPRI), 2Kansai Research Center,Forestry and Forest Products Research Institute (FFPRI), 3Department of Wood Properties and Processing,Forestry and Forest Products Research Institute (FFPRI), 4Faculty of Agriculture,Kyushu University, 5Department of Bacteriology,University of Wisconsin, 6Research Faculty of Agriculture,Hokkaido University
Chapters
- 00:04Title
- 01:14Sampling: Tension Relaxation within Water Column of Xylem Conduits
- 03:00Freeze Fixation with Liquid Nitrogen
- 04:04Specimen Preparation
- 05:49Transfer to the Cryo-SEM Specimen Chamber
- 06:30Freeze-etching
- 07:00Results: Xylem Water Distribution in Woody Plants
- 09:04Conclusion
Körte su dağılımının gözlemlenmesi, odunsu bitkilerde suyun akış dinamikleri ile ilgili önemli bilgiler sağlar. Bu çalışmada, örnek hazırlama sırasında su durumundaki artifaktüel değişiklikleri ortadan kaldıran bir kriyostat ve Cryo-SEM kullanarak situ 'da xylem su dağıtımını gözlemlemek için pratik bir yaklaşım gösteririz.
Tags
XylemWater DistributionCryo-scanning Electron MicroscopeFreeze-fixationXylem TensionWater PotentialCavitationXylem CellsAerotomeWoody PlantsWater RegimeSeasonal VariationBiotic StressFreeze/thaw CycleWet WoodSampling ProcedurePressure ChamberRelaxation TreatmentWater SurfaceTranspirationVessel Length
