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分离叶测定,以简化马铃薯中基因表达研究,在咀嚼昆虫曼杜卡性塔感染
JoVE Journal
Environment
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Environment
Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta
Please note that all translations are automatically generated.
Click here for the English version.
分离叶测定,以简化马铃薯中基因表达研究,在咀嚼昆虫曼杜卡性塔感染
DOI:
10.3791/59153-v
•
05:56 min
•
May 15, 2019
•
Nicole G. Novak
,
Frances G. Perez
,
Richard W. Jones
,
Susan D. Lawrence
1
Invasive Insect Biocontrol and Behavior Lab
,
United States Department of Agriculture (USDA), Agricultural Research Service (ARS)
,
2
Genetic Improvement for Fruits and Vegetables Lab
,
USDA-ARS
Chapters
00:04
Title
01:02
Potato Plant and Insect Preparation
02:02
Infestation Experiment Setup
03:14
Infestation and Leaf Harvest
04:16
Results: Representative Leaf Damage and Gene Expression
05:23
Conclusion
Summary
Automatic Translation
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Automatic Translation
该方法通过将
曼杜卡性幼虫
应用于马铃薯分离叶,创造出天然的草食性植物组织受损。植物组织被测定为六个转录因子同源的表达参与早期反应昆虫食草动物。
Tags
Detached Leaf Assay
Gene Expression
Potato
Manduca Sexta
Insect Herbivory
Chewing Insect
Nodal Propagation
Larval Stage
Plant-insect Interaction
Reproducible Data
Plant Genetic Improvement
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