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Journal
/
Biology
/
一种用于阴
南芥
叶片安装的通用方法
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
A Versatile Method for Mounting
Arabidopsis
Leaves for Intravital Time-lapse Imaging
Please note that all translations are automatically generated.
Click here for the English version.
一种用于阴
南芥
叶片安装的通用方法
DOI:
10.3791/59147-v
•
07:52 min
•
February 11, 2019
•
Shigeyuki Betsuyaku
2,3,4
,
Nobuhiko Nomura
4
,
Hiroo Fukuda
1
Japan Science and Technology Agency (JST), PRESTO
,
2
Department of Biological Sciences, Graduate School of Science
,
The University of Tokyo
,
3
Faculty of Life and Environmental Sciences
,
University of Tsukuba
,
4
Microbiology Research Center for Sustainability
,
University of Tsukuba
Chapters
00:04
Title
00:28
Preparation of Plants and Pathogens
01:48
Pathogen Inoculation
03:10
Mounting the Inoculated Leaf
05:02
Microscopic Time-lapse Observation
06:19
Results: Intravital Time-lapse Imaging of Mounted
Arabidopsis
Leaves
07:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们报告了一种简单和通用的方法, 用于在较长时间内
对拟南芥
叶片进行荧光实时成像。我们使用转基因
拟南芥
植物表达荧光记者基因在免疫相关的启动子控制下作为一个例子, 以获得时空了解植物免疫反应。
Tags
Arabidopsis Leaves
Intravital Time-lapse Imaging
Spatiotemporal Regulation
Gene Of Interest
Immunity
Promoter Activity
Pathogen Inoculation
Bacterial Suspension
Fluorescence Stereomicroscope
PR1 Promoter
Article
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