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Immunology and Infection
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高盐抗原表达树突状细胞的分离和采用转移
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Immunology and Infection
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Immunology and Infection
Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells
Please note that all translations are automatically generated.
Click here for the English version.
高盐抗原表达树突状细胞的分离和采用转移
DOI:
10.3791/59124-v
•
09:29 min
•
March 05, 2019
•
Justin P. Van Beusecum
,
Liang Xiao
,
Natalia R. Barbaro
,
David M. Patrick
,
Annet Kirabo
1
Division of Clinical Pharmacology, Department of Medicine
,
Vanderbilt University Medical Center
Chapters
00:04
Title
00:52
Isolation of Spleens from Mice
01:27
Generation of Single Cell Suspensions from Spleens
02:39
Isolation of CD11c
+
DCs from Splenic Single Cell Suspension
03:41
Magnetic Separation of CD11c
+
DCs
04:51
Adoptive Transfer of High Salt Treated CD11c
+
DCs
06:56
Results: Analysis of the High Salt Treated Antigen-presenting Dendritic Cells
08:52
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们提出了一个协议, 以分离树突状细胞从小鼠的脾脏通过磁性细胞分类和随后采用转移到天真的小鼠。选择高盐活化树突状细胞模型, 解释采用转移和流式细胞术的分步过程。
Tags
Dendritic Cells
CD11c
Hypertension
Cardiovascular Disease
Adoptive Transfer
Spleen
Isolation
Collagenase D
DNase
MACS
Flow Cytometry
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