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Cancer Research
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三维细胞培养中胰腺肌囊内增生的模拟与操纵
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Mimicking and Manipulating Pancreatic Acinar-to-Ductal Metaplasia in 3-dimensional Cell Culture
Please note that all translations are automatically generated.
Click here for the English version.
三维细胞培养中胰腺肌囊内增生的模拟与操纵
DOI:
10.3791/59096-v
•
08:16 min
•
February 11, 2019
•
Alicia K. Fleming Martinez
,
Peter Storz
1
Department of Cancer Biology
,
Mayo Clinic Florida
Chapters
00:04
Title
00:30
Acinar Cell Isolation
05:11
Viral Infection
05:50
Embedment of Cells in Collagen and Basement Membrane Matrix
07:12
Results: Primary Acinar Cells Plated within Collagen or Basement Membrane Matrix after Adenoviral Infection and Stimulation with TGF–α
07:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
原代腺细胞分离、蛋白表达或活性调节、培养和下游应用在腺尖囊转牙 (adm) 的体外研究中非常有用, 这是胰腺癌发展的早期事件。
Tags
Pancreatic Acinar-to-ductal Metaplasia
3D Cell Culture
Real-time Visualization
Cell Signaling Studies
Pancreas Dissection
Collagenase Digestion
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