Journal
/
Medicine
/
增殖性糖尿病视网膜病变纤维血管并发症的体外组织培养模型
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
An Ex Vivo Tissue Culture Model for Fibrovascular Complications in Proliferative Diabetic Retinopathy
Please note that all translations are automatically generated. Click here for the English version.

增殖性糖尿病视网膜病变纤维血管并发症的体外组织培养模型

DOI:
10.3791/59090-v

•

08:10 min

•

January 25, 2019

•

, , ,
1Research Programs Unit, Genome-Scale Biology, Biomedicum Helsinki,University of Helsinki, 2Unit of Vitreoretinal Surgery, Ophthalmology,University of Helsinki and Helsinki University Hospital, 3Department of Microbiology, Tumor, and Cell Biology (MTC),Karolinska Institutet

Chapters

  • 00:04Title
  • 00:40Fibrovascular Tissue Dissection
  • 01:37Casting Upright Fibrin Gel Droplets for the Characterization of Native FT and Ex Vivo Culture
  • 02:52Native FT and Ex Vivo Culture End-Point
  • 03:19Whole-Mount Immunofluorescence Staining
  • 07:00Results: PDR Ex Vivo Culture Induced Sprouting of CD31-positive Endothelium and Vasculature Preservation in Response to VEGFA
  • 07:31Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们提出了一个方案, 研究增殖性糖尿病视网膜病变的病理生理学使用患者衍生的, 手术切除, 纤维血管组织的三维本地组织表征和体外培养。这种体外培养模型也适合测试或开发新的治疗方法。

Tags

Ex VivoFibrovascularProliferative Diabetic RetinopathyMicrovascular ComplicationDiabetic RetinopathyTransconjunctival Microincision Vitreoretinal SurgeryFibrinogenFibrin GelEx Vivo Culture MediumParaformaldehydePBSSodium Azide

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

患者组织及基因传递考试前的体外培养

前溶瘤病毒的活组织的体内感染

一个<em>体外</em>模型在乳腺癌研究激素的作用

人掌腱膜挛缩的<em>离体</em>文化为肌成纤维细胞的研究和细胞外基质相互作用

创作和移植的糖尿病伤口愈合模型中脂肪源性干细胞 (ASC) 表

母乳促进 Enteroids 的生长: 一个前体细胞增殖模型

体外常温肝灌注的小动物模型

心内神经元对体外 Langendorff 系统心脏电生理和心脏交感神经的影响

创面愈合研究的前活体角膜器官培养模型

糖尿病家兔欣德肢体缺血的临床前模型

Read Article