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ヒトP53を研究する機能アッセイを開発するシャーシとしての酵母
JoVE Journal
Cancer Research
Author Produced
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JoVE Journal Cancer Research
Yeast As a Chassis for Developing Functional Assays to Study Human P53
DOI:

14:57 min

August 04, 2019

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Chapters

  • 00:00Title
  • 00:54Construction of ADE2 or LUC1 Reporter Yeast Strain that Contains a Specific RE (yAFM-RE or yLFM-RE)
  • 03:06Evaluation of P53 Protein Transactivation Ability using the Qualitative Color-based ADE2 Yeast Assay
  • 04:27Evaluation of P53 Protein Transactivation Ability using the Quantitative Luminescence-based LUC1 Yeast Assay
  • 07:06Evaluation of P53 Protein Growth Inhibition using the Yeast Phenotypic Assay
  • 08:08Results
  • 13:51Conclusion

Summary

Automatic Translation

ここでは、ヒトP53トランス活性化の可能性、その様々な癌関連変異の影響、共発現相互作用タンパク質、および酵母サッカロマイセスセレビシエレポーター株を構築し、利用するための4つのプロトコルを示します。特定の低分子の効果。

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