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爬行动物皮肤脂类的化学分离、定量和分离
JoVE Journal
Environment
This content is Free Access.
JoVE Journal
Environment
Chemical Isolation, Quantification, and Separation of Skin Lipids from Reptiles
Please note that all translations are automatically generated.
Click here for the English version.
爬行动物皮肤脂类的化学分离、定量和分离
DOI:
10.3791/59018-v
•
07:55 min
•
February 07, 2019
•
Paige E. Baedke
,
Holly R. Rucker
,
Robert T. Mason
,
M. Rockwell Parker
1
Department of Biology
,
James Madison University
,
2
Department of Integrative Biology
,
Oregon State University
Chapters
00:04
Title
00:37
Shed Skin Extraction and Lipid Mass Determination
03:01
Chromatography Column Preparation
05:19
Lipid Extract Fractionation
06:29
Results: Representative Standardized Fraction Masses Following Elution
07:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在爬行动物中, 来自中性的皮肤脂质对于性信号至关重要, 有可能用于入侵物种管理。在这里, 我们描述了从脱落的皮肤或整个动物中提取皮肤脂质, 确定和分析总脂质, 并
通过
柱层析分离脂质的方案。
Tags
Chemical Isolation
Skin Lipids
Reptiles
Chemical Communication
Bioactive Signaling Molecules
Hexane Extraction
Rotary Evaporation
Lipid Quantification
Chromatography
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