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atp 利用商业检测和染色法定量检测淋病奈瑟菌骨料的抗生素敏感性
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining
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atp 利用商业检测和染色法定量检测淋病奈瑟菌骨料的抗生素敏感性

DOI:
10.3791/58978-v

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08:04 min

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February 08, 2019

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, , , , ,
1Department of Marine Biotechnology and Resources,National Sun Yat-Sen University, 2Department of Cell Biology and Molecular Genetics,University of Maryland

Chapters

  • 00:04Title
  • 00:47Neisseria gonorrhoeae (GC) Strain Maintenance
  • 01:31GC Aggregate Quantification
  • 03:13GC Live/Dead Aggregate Analysis
  • 04:26Image Analysis
  • 05:48Results: Representative GC Aggregation Survival Before and After Antibiotic Treatment
  • 07:21Conclusion

Summary

Automatic Translation

Automatic Translation

采用简单的 atp 测量法和活死染色法对头孢曲松治疗后淋病奈瑟菌的生存率进行了定量和可视化。该方案可以扩展到检查任何抗生素的抗菌作用, 并可用于确定细菌生物膜中抗生素的最小抑制浓度。

Tags

Neisseria GonorrhoeaeAntibiotic SusceptibilityATP-utilization AssayLive/dead StainingBacterial AggregatesCeftriaxoneMicrobial CultureOptical DensitySonication

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