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Journal
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Immunology and Infection
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一种基于板的大鼠胎盘自然杀伤细胞细胞细胞溶解功能评价的细胞毒性测定
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Immunology and Infection
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Immunology and Infection
A Plate-based Cytotoxicity Assay for the Assessment of Rat Placental Natural Killer Cell Cytolytic Function
Please note that all translations are automatically generated.
Click here for the English version.
一种基于板的大鼠胎盘自然杀伤细胞细胞细胞溶解功能评价的细胞毒性测定
DOI:
10.3791/58961-v
•
10:44 min
•
June 02, 2019
•
Cedar H. Baik
,
Mallory Geer
,
Olivia K. Travis
,
Denise C. Cornelius
2
1
Department of Emergency Medicine
,
University of Mississippi Medical Center
,
2
Department of Experimental Therapeutics and Pharmacology
,
University of Mississippi Medical Center
Chapters
00:04
Title
01:28
Lymphocyte Cell Isolation from Placentas
02:28
Isolation of Natural Killer Cells
06:32
Cytotoxicity Assay: Retrieving NK Cells or YAC1 from Culture or Passing Cells
07:51
Cytotoxicity Assay: Assay Protocol
09:17
Results: Assessment of the Cytotoxicity Assay
09:56
Conclusion
Summary
Automatic Translation
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在这里, 我们提供了一个详细的方法, 以分离和评估细胞毒性功能的自然杀伤细胞从胎盘通过比色板分析。选择减少子宫灌注压力大鼠胎盘缺血模型, 以证明其抗体介导的分离和对自然杀伤细胞细胞毒性功能的评估。
Tags
– Plate-based Cytotoxicity Assay- Rat Placental Natural Killer Cells- Cytolytic Function- Cytotoxic Activity- Inexpensive Method- Specialized Equipment- Hazardous Materials- CD8+ Cytolytic Cells- Target-effector Cell Ratio Optimization- Mononuclear Cell Isolation- Placenta Homogenization- Density Gradient Centrifugation- Biotin-labeled CD3 Antibody- Magnetic Bead Separation
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