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利用微阵列研究微环境对癌症细胞表型的影响
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Using Microarrays to Interrogate Microenvironmental Impact on Cellular Phenotypes in Cancer
DOI:

08:20 min

May 21, 2019

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Chapters

  • 00:04Title
  • 00:47Printing Microenvironment Microarrays (MEMAs) Using an Array Printing Robot
  • 01:37Creation of Ligand Treatment Plates
  • 02:28Culturing Cells on MEMAs
  • 04:23Fixing, Staining, and Imaging MEMAs
  • 06:14Results: Cell Cycle Profiles, Cell Number and EdU Incorporation
  • 07:44Conclusion

Summary

Automatic Translation

这里介绍的方法的目的是展示如何制造微环境微阵列 (MEMA) 并用于询问数千种简单组合微环境对培养细胞表型的影响。

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