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利用微阵列研究微环境对癌症细胞表型的影响
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Cancer Research
Using Microarrays to Interrogate Microenvironmental Impact on Cellular Phenotypes in Cancer
Please note that all translations are automatically generated.
Click here for the English version.
利用微阵列研究微环境对癌症细胞表型的影响
DOI:
10.3791/58957-v
•
08:20 min
•
May 21, 2019
•
Rebecca Smith
,
Kaylyn Devlin
,
David Kilburn
,
Sean Gross
,
Damir Sudar
,
Elmar Bucher
,
Michel Nederlof
,
Mark Dane
,
Joe W. Gray
,
Laura Heiser
,
James E. Korkola
1
Department of Biomedical Engineering, Knight Cancer Institute
,
Oregon Health and Science University
,
2
Quantitative Imaging Systems LLC
Chapters
00:04
Title
00:47
Printing Microenvironment Microarrays (MEMAs) Using an Array Printing Robot
01:37
Creation of Ligand Treatment Plates
02:28
Culturing Cells on MEMAs
04:23
Fixing, Staining, and Imaging MEMAs
06:14
Results: Cell Cycle Profiles, Cell Number and EdU Incorporation
07:44
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里介绍的方法的目的是展示如何制造微环境微阵列 (MEMA) 并用于询问数千种简单组合微环境对培养细胞表型的影响。
Tags
Microarray
Microenvironment
Tumor
Phenotype
MEMA Platform
Extracellular Matrix
Microenvironment Microarrays
Collagen
Ligand
Cell Culture
Blocking Buffer
PBS
MCF7 Cells
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