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Journal
/
Immunology and Infection
/
化学
抑制剂库筛选 t 细胞受体信号的中介识别
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Identification of Mediators of T-cell Receptor Signaling
via
the Screening of Chemical Inhibitor Libraries
Please note that all translations are automatically generated.
Click here for the English version.
化学
抑制剂库筛选 t 细胞受体信号的中介识别
DOI:
10.3791/58946-v
•
08:49 min
•
January 22, 2019
•
Elijah W. Chen*
1
,
Chyan Ying Ke*
2,3
,
Joanna Brzostek*
1
,
Nicholas R. J. Gascoigne
,
Vasily Rybakin
4
1
Department of Microbiology and Immunology, Yong Loo Lin School of Medicine
,
National University of Singapore
,
2
Singapore Immunology Network
,
A*STAR
,
3
Curiox Biosystems
,
4
Department of Immunobiology, Rega Institute for Medical Research
,
Katholieke Universiteit (KU) Leuven
Chapters
00:04
Title
01:15
Kinase Inhibitor Library Screening (Centrifuge-independent Assay)
02:15
Stimulation of Thymocytes Using Anti-CD3/CD28 Beads
03:52
Staining of Surface Antigens and Fixation of Cells
05:09
Intracellular Staining for Active Caspase 3
06:05
Results: Flow-Cytometry-Based Screening of Modulators of T-cell Receptor Signaling and T-cell Activation
07:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文采用基于流式细胞仪的检测方法筛选化学抑制剂文库, 用于鉴定影响 t 细胞受体信号转导的抑制剂及其靶点。此处描述的方法也可以扩展为高通量筛选。
Tags
T-cell Receptor Signaling
Chemical Inhibitor Libraries
High-throughput Assay
TCR Signaling Mediators
Immune Diseases
Thymocytes
Kinase Inhibitors
Anti-CD3/CD28 Beads
Apoptosis
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