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Journal
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Cancer Research
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荧光肽酶谱法检测蛋白酶活性
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Detection of Protease Activity by Fluorescent Peptide Zymography
Please note that all translations are automatically generated.
Click here for the English version.
荧光肽酶谱法检测蛋白酶活性
DOI:
10.3791/58938-v
•
09:56 min
•
January 20, 2019
•
Ameya A. Deshmukh
2
,
Jessica L. Weist
,
Jennifer L. Leight
2
1
Comprehensive Cancer Center, James Cancer Hospital and Solove Research Center
,
Ohio State University
,
2
Department of Biomedical Engineering, College of Engineering
,
Ohio State University
Chapters
00:04
Title
01:03
Preparation of the Resolving Gel Layer
01:52
Preparation of the Azido-PEG3-maleimide Linker Molecule
03:31
Preparation of the Peptide Resolving Gel Layer
05:16
Preparation of the Stacking Gel
06:05
Electrophoresis of Biological Samples in Peptide Zymography Gels
07:26
Results: Detection of Protease Activity
09:15
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个详细的协议, 修改酶图技术, 其中荧光肽被用作可降解的底物, 而不是原生蛋白质。生物样品在荧光肽酶谱中的电泳使检测到比以前的酶学技术更广泛的蛋白酶。
Tags
Protease Activity
Fluorescent Peptide Zymography
Fluorescent Peptides
Degradable Substrate
Modular Design
Biomaterial Degradation
Polyacrylamide Gel
Azido-PEG3-Maleimide
Inert Gas
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