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白
念珠
菌实时细胞外通量实时分析的生物能量学研究
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Medicine
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JoVE Journal
Medicine
Bio-energetics Investigation of
Candida albicans
Using Real-time Extracellular Flux Analysis
Please note that all translations are automatically generated.
Click here for the English version.
白
念珠
菌实时细胞外通量实时分析的生物能量学研究
DOI:
10.3791/58913-v
•
08:48 min
•
March 19, 2019
•
Sundararajan Venkatesh*
1
,
Manju Chauhan*
2
,
Carolyn Suzuki
,
Neeraj Chauhan
2
1
Department of Microbiology, Biochemistry and Molecular Genetics, New Jersey Medical School, Rutgers
,
The State University of New Jersey
,
2
Public Health Research Institute, Rutgers
,
The State University of New Jersey
Chapters
00:04
Title
00:48
Coating of the Assay Plate with Poly-D-Lysine
01:53
Hydration of Sensor Cartridge
02:33
Growing and Seeding Cells in the Poly-D-Lysine-coated Plates
03:33
Assay Protocol
05:56
Results: Real-time Extracellular Flux Analysis
07:59
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个逐步的协议, 以研究线粒体呼吸和糖酵解功能在
白色念珠菌使用一个
额外的通量分析仪。
Tags
Candida Albicans
Bio-energetics
Extracellular Flux Analysis
Mitochondrial Respiration
Glycolytic Function
Fungal Pathogens
Poly-D-Lysine
Sensor Cartridge
YPD Broth
Assay Medium
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