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Immunology and Infection
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流式细胞术测定小鼠胸腺、胰腺干淋巴结和脾脏的调节 t 细胞亚群
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Determination of Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node and Spleen Using Flow Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
流式细胞术测定小鼠胸腺、胰腺干淋巴结和脾脏的调节 t 细胞亚群
DOI:
10.3791/58848-v
•
08:06 min
•
February 27, 2019
•
Zhengkang Luo
,
Lina Thorvaldson
,
Martin Blixt
,
Kailash Singh
1
Department of Medical Cell Biology
,
Uppsala University
Chapters
00:04
Title
00:29
Harvesting Organs from Animals
01:05
Single Cell Isolation from Thymus and Spleen
02:41
Single Cell Isolation from PDLN
03:58
Flow Cytometry
06:07
Results: Determination of the Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node, and Spleen
07:36
Conclusion
Summary
Automatic Translation
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在此, 我们提出了一个方案, 以准备单细胞从小鼠胸腺, 胰腺引流淋巴结和脾脏, 进一步研究这些细胞使用流式细胞仪。此外, 该协议还用于使用流式细胞仪确定调节 t 细胞的子集。
Tags
Flow Cytometry
Regulatory T Cells
Thymus
Pancreatic Draining Lymph Node
Spleen
Immune Cells
Cell Isolation
Cell Suspension
Cell Lysis
Cell Quantification
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