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Environment
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在体外
显示砷对皮肤细胞迁移影响的划痕测定
JoVE Journal
Environment
This content is Free Access.
JoVE Journal
Environment
In Vitro
Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration
Please note that all translations are automatically generated.
Click here for the English version.
在体外
显示砷对皮肤细胞迁移影响的划痕测定
DOI:
10.3791/58838-v
•
09:24 min
•
February 23, 2019
•
Bronson I. Pinto
4
,
Nathan D. Cruz
,
Oscar R. Lujan
,
Catherine R. Propper
3
,
Robert S. Kellar
2,4
1
Department of Biological Sciences
,
Northern Arizona University
,
2
Department of Mechanical Engineering
,
Northern Arizona University
,
3
Department of Chemistry and Biochemistry
,
Northern Arizona University
,
4
Center for Bioengineering Innovation
,
Northern Arizona University
Chapters
00:04
Title
01:05
Human Dermal Fibroblasts Seeding in a T75 Flask
02:37
Cell Counting and Subculture into 12-Well Tissue Culture Plate
06:11
Scratch Assay Technique and Arsenic Contamination
07:44
Results: Analysis of the Scratch Assay
08:39
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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Automatic Translation
本研究的重点是伤口愈合的
体外
模型 (划痕检测), 作为一种机制, 以确定环境污染物, 如砷如何影响细胞迁移。结果表明, 这种
体外
检测在
体内
实验之前为细胞迁移的变化提供了快速和早期的迹象。
Tags
In Vitro Scratch Assay
Cell Migration
Wound Healing
Arsenic
Skin Cells
Cell Culture
Cell Migration Assay
Cell Monolayer
Cell Line
Cell Suspension
Cell Density
Article
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