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卡瑞津、S100 蛋白和蛋白质基因产物9.5 的免疫修饰直肠吸吮活检诊断
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Neuroscience
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JoVE Journal
Neuroscience
Diagnosis of Hirschsprung’s Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5
Please note that all translations are automatically generated.
Click here for the English version.
卡瑞津、S100 蛋白和蛋白质基因产物9.5 的免疫修饰直肠吸吮活检诊断
DOI:
10.3791/58799-v
•
05:45 min
•
April 26, 2019
•
Shuiqing Chi
,
Mijing Fang
,
Kang Li
,
Li Yang
,
Shao-tao Tang
1
Department of Pediatric Surgery, Union Hospital, Tongji Medical College
,
Huazhong University of Science and Technology
,
2
Division of Gastroenterology, Hepatology and Nutrition
,
Cincinnati Children’s Hospital Medical Center
Chapters
00:04
Title
00:40
Rectal Suction Biopsy (RSB) Section Preparation
01:52
Antigen Retrieval and Tissue Staining
04:25
Results: Representative RSB Immunohistochemical Analysis
05:09
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该方案描述了对卡莱丁、S100 蛋白和蛋白质基因产物9.5 进行免疫染色直肠抽吸活检的过程。这种新型辅助诊断方法对先天性巨结肠病具有较好的敏感性和特异性。
Tags
Hirschsprung’s Disease
Immunostaining
Rectal Suction Biopsy
Calretinin
S100 Protein
Protein Gene Product 9.5
Diagnosis
Sensitivity
Specificity
Paraformaldehyde
Dehydration
Paraffin Embedding
Microtome
Antigen Retrieval
Hydrogen Peroxide
Bovine Serum Albumin
Primary Antibody
Polymer Enhancer
Secondary Antibody
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