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Immunology and Infection
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小鼠边缘区 b 细胞剪切流诱导迁移的体外分析
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Immunology and Infection
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Immunology and Infection
Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro
Please note that all translations are automatically generated.
Click here for the English version.
小鼠边缘区 b 细胞剪切流诱导迁移的体外分析
DOI:
10.3791/58759-v
•
08:31 min
•
November 26, 2018
•
Kerry Tedford
,
Laura Tech
,
Michael Steiner
,
Mark Korthals
,
Klaus-Dieter Fischer
1
Institute of Biochemistry and Cell Biology
,
OVGU University of Magdeburg
Chapters
00:04
Title
00:43
Preparing the 6-chambered Slides for the Flow Experiment
01:49
Fluidics Unit Preparation
03:07
Cell Loading
04:04
Attaching the Fluidics Unit and Imaging the Cells
05:28
Migration Track Analysis
07:02
Results: Quantifying Marginal Zone B-cell Shuttling
08:00
Conclusion
Summary
Automatic Translation
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Automatic Translation
边缘区 b 细胞 (mzb) 通过调整其迁移路径的方向来响应剪切流的力。该协议演示了如何使用流体单元、泵、显微镜成像系统和自由软件记录和分析迁移。
Tags
Shear Flow
Marginal Zone B Cells
Migration Assay
ICAM-1
Leukocytes
Cytoskeleton
Integrin Ligands
Chemokines
Activated T Cells
Fluid Pump
Migration Buffer
Shear Stress
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