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Journal
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Immunology and Infection
/
增强型绿色荧光蛋白表达
大肠杆菌
评价小鼠腹腔巨噬细胞吞噬细胞增多
JoVE Journal
Immunology and Infection
Author Produced
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JoVE Journal
Immunology and Infection
Using Enhanced Green Fluorescence Protein-expressing
Escherichia Coli
to Assess Mouse Peritoneal Macrophage Phagocytosis
Please note that all translations are automatically generated.
Click here for the English version.
增强型绿色荧光蛋白表达
大肠杆菌
评价小鼠腹腔巨噬细胞吞噬细胞增多
DOI:
10.3791/58751-v
•
12:35 min
•
January 04, 2019
•
Yu Zhang *
1
,
Guan Wang*
1
,
Jia Lu
,
Li-ming Xu
,
Jun-yu Xiong
1
Anesthesiology Department
,
The Second Hospital of Dalian Medical University
,
2
Scientific Research Center
,
The Second Hospital of Dalian Medical University
Chapters
00:00
Title
01:40
Construct the pET-SUMO-EGFP Plasmid and Induce EGFP-expression
04:28
Mouse Peritoneal Macrophage Isolation and Primary Culture
06:57
Macrophage Phagocytosis Assay using Flourescence Microscopy
08:28
Macrophage Phagocytosis Assay using Flow Cytometer
11:05
Results
11:45
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个评估小鼠腹腔巨噬细胞吞噬使用增强绿色荧光蛋白表达大肠杆菌的方案.
Tags
EGFP-expressing Escherichia Coli
Phagocytosis
Macrophages
Peritoneal Macrophages
Innate Immune Function
Aging
Fluorescence Microscopy
Flow Cytometry
TA Cloning
PET SUMO Vector
BL21 Competent Cells
Kanamycin
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