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淋巴瘤组织学标本中多路荧光组织化学染色、成像与分析
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Immunology and Infection
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JoVE Journal Immunology and Infection
Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
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淋巴瘤组织学标本中多路荧光组织化学染色、成像与分析

DOI:
10.3791/58711-v

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07:52 min

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January 09, 2019

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, , , , , , , , ,
1Department of Laboratory Medicine,AnSteel Group General Hospital, 2Cancer Science Institute of Singapore,National University of Singapore, 3Department of Pathology, Yong Loo Lin School of Medicine,National University of Singapore, 4Department of Haematology-Oncology,National University Health System

Chapters

  • 00:04Title
  • 00:57Monoplex Tyramide-Based Immunofluorescence
  • 03:22Monoplex Slide and Tissue Microarray (TMA) Scanning
  • 04:36Data Analysis
  • 06:28Results: Representative Mutiplexed Fluorescent Immunohistochemical (mf-IHC) Staining, Imaging, and Analysis
  • 07:10Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们描述了一个协议的多重荧光免疫组化染色和成像同时定位的多种癌症相关抗原在淋巴瘤。该协议可推广到所有组织切片中生物标志物的共化分析。

Tags

Multiplexed Fluorescent ImmunohistochemistryLymphomaTumor MicroenvironmentAntigen Co-expressionSpatial RelationshipAntigen RetrievalMicrowave StrippingPeroxidase BlockTyramide-based Fluorescent ReagentSpectral Imaging

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