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Journal
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Immunology and Infection
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在研究
隐球菌-
阿米巴相互作用中,显微技术和荧光读数的补充应用
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying
Cryptococcus
-Amoeba Interactions
Please note that all translations are automatically generated.
Click here for the English version.
在研究
隐球菌-
阿米巴相互作用中,显微技术和荧光读数的补充应用
DOI:
10.3791/58698-v
•
10:56 min
•
June 22, 2019
•
Uju L. Madu
,
Olihile M. Sebolai
1
Department of Microbial, Biochemical and Food Biotechnology
,
University of the Free State
Chapters
00:00
Title
00:40
Cultivation and Standardization of Fungal Cells
01:54
Cultivation and Standardization of Amoeba Cells
02:34
Fluorescence Staining of Cells to Study Phagocytosis
04:50
Usage of Transmission Electron Microscopy to Study Phagocytosis
07:02
Results
09:31
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文详细介绍了利用静止光图像和高分辨率透射电子显微镜图像进行制备隐球细胞和阿米巴共同培养的协议。此处说明定量数据如何补充此类定性信息。
Tags
Microscopic Techniques
Fluorescence Reading
Cryptococcus-Amoeba Interactions
Phagocytosis
Pathogenic Fungus
Cryptococcus Neoformans
Environmental Predator
Amoeba
Confocal Laser-scanning Microscopy
Transmission Electron Microscopy
YPD Agar
YNB Broth
Glucose
Amoeba Cells
Peptone Yeast Extract Glucose Broth
Trypan Blue
Fluorescein Isothiocyanate
PBS
Glutaraldehyde
Co-culture
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