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用病毒载体接种后, mhc i 四分体染色同时定量抗载体和抗转基因特异性 cd8
+
t 细胞
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8
+
T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector
Please note that all translations are automatically generated.
Click here for the English version.
用病毒载体接种后, mhc i 四分体染色同时定量抗载体和抗转基因特异性 cd8
+
t 细胞
DOI:
10.3791/58680-v
•
08:10 min
•
November 28, 2018
•
Sarah Wilmschen
,
Zoltan Banki
,
Dorothee von Laer
,
Janine Kimpel
1
Division of Virology, Department of Hygiene, Microbiology, Social Medicine
,
Medical University of Innsbruck
Chapters
00:04
Title
00:49
Sample Collection
01:21
Staining Set-up
01:59
Tetramer Staining and Preparation of Antibodies
03:17
Staining of Samples
04:24
Lysis of Erythrocytes
05:09
Flow Cytometric Measurement and Analysis
06:53
Results: Quantification of Antigen-specific CD8
+
T Cells in Mouse Blood of Lymphoid Organs
07:36
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个协议的体外定性检测抗原特异性 cd8
+ t 细胞
。从器官或少量血液中进行单细胞悬浮液分析是可能的。广泛的研究需要分析细胞毒性 t 细胞的反应 (疫苗接种和癌症免疫治疗研究)。
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