/
/
Flusso Cytometric Detection neonata al seno cancro cellule staminali-come delle cellule dopo l'inversione di apoptosi
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1School of Life Sciences,The Chinese University of Hong Kong, 2State Key Laboratory of Agrobiotechnology,The Chinese University of Hong Kong, 3Key Laboratory for Regenerative Medicine, Ministry of Education,The Chinese University of Hong Kong, 4Centre for Novel Biomaterials,The Chinese University of Hong Kong
Chapters
- 00:04Title
- 01:08Preparation of Breast Non-stem Cancer Cells
- 05:08Apoptotic Induction and Detection
- 07:17Isolation of Apoptotic Cells and Apoptosis Reversal Procedure
- 08:22Measurement of Breast CSC-like Cells by Flow Cytometry
- 09:11Results: CSC-like Cells Transited from Breast Non-stem Cancer Cells During Apoptosis Reversal
- 10:29Conclusion
Qui, presentiamo un protocollo per isolare cellule di carcinoma mammario apoptotica ordinando di fluorescenza-attivato delle cellule e ulteriormente rilevare la transizione delle cellule staminali non cancro al seno al seno cancro cellule staminali-come le cellule dopo inversione di apoptosi mediante citometria a flusso.
