JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
小鼠肝脏消化对淋巴内皮细胞的流式细胞仪分析
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells
Please note that all translations are automatically generated.
Click here for the English version.
小鼠肝脏消化对淋巴内皮细胞的流式细胞仪分析
DOI:
10.3791/58621-v
•
08:07 min
•
January 07, 2019
•
Jeffrey M. Finlon
,
Matthew A. Burchill
,
Beth A. Jirón Tamburini
2
1
Division of Gastroenterology and Hepatology, Department of Medicine
,
University of Colorado Anschutz Medical Campus, School of Medicine
,
2
Department of Immunology and Microbiology
,
University of Colorado Anschutz Medical Campus
Chapters
00:04
Title
00:52
Liver Single-cell Suspension Preparation
03:36
Flow Cytometric Analysis
05:49
Results: Representative Murine Liver Lymphatic Endothelial Cell (LEC) Characterization
07:22
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该方案的目标是使用所述标记识别肝脏内的淋巴管内皮细胞群。我们利用胶原酶 iv 和 dnase 和组织的温和切碎, 结合流式细胞仪, 以确定一个独特的淋巴内皮细胞的群体。
Tags
Liver
Lymphatic Endothelial Cells
Flow Cytometry
Single-cell Suspension
Liver Biology
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
流式细胞仪分析小鼠主动脉内流的免疫细胞
主要鼠Ⅱ型肺泡上皮细胞的功能及分子生物学研究的流式细胞仪分离
非侵入性光学成像淋巴血管的鼠标
分离和流式细胞仪分析从缺血小鼠脑免疫细胞
分离和胶质瘤浸润外周血单个核细胞的流式细胞仪分析
小鼠小肠淋巴细胞的分离和流式细胞表征
粒子结合Bet v 1的变应原PM10的流式细胞分析
人全血中NK细胞杀伤活性的流式细胞仪分析
人类宫颈的γδT细胞的分离和流式细胞仪分析
全骨髓的制备,用于中性粒系细胞的大规模细胞测定分析
Read Article