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Neuroscience
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微胶质细胞抗体介导的陶氏清除研究
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Cell-based Assay to Study Antibody-mediated Tau Clearance by Microglia
Please note that all translations are automatically generated.
Click here for the English version.
微胶质细胞抗体介导的陶氏清除研究
DOI:
10.3791/58576-v
•
07:18 min
•
November 09, 2018
•
Donata De Marco
,
Renske Taggenbrock
3
,
Rosa Crespo
,
Wouter Koudstaal
,
Elizabeth Ramsburg
,
Adrian Apetri
1
Neuroscience Discovery
,
Janssen Pharmaceutical Company of Johnson & Johnson, Belgium
,
2
Department of Hematopoiesis
,
Sanquin Research and Landsteiner Laboratory, The Netherlands
,
3
Janssen Prevention Center
,
Janssen Pharmaceutical Company of Johnson & Johnson, The Netherlands
,
4
Neuroscience Discovery
,
Janssen Pharmaceutical Company of Johnson & Johnson, Pennsylvania
Chapters
00:04
Title
00:37
Uptake Assay with Fluorescence-activated Cell Sorting (FACS) Read-out
04:33
FACS Analysis
05:41
Results: Antibody-mediated Increase of Tau Uptake in BV-2 Cells
06:45
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们描述了一种基于细胞的检测方法, 以定量评估小胶质细胞对 tau 的吸收, 目的是创建一个研究工具, 以更好地描述抗 tau 抗体的作用机制。
Tags
Cell-based Assay
Antibody-mediated Tau Clearance
Microglia
Alzheimer’s Disease
PH Dye-Tau Aggregates
Anti-Tau Antibodies
BV-2 Cells
Cell Culture
Trypsin EDTA
Centrifugation
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