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在体外
陶氏蛋白聚集与药物筛选研究方法
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
In Vitro
Assay for Studying the Aggregation of Tau Protein and Drug Screening
Please note that all translations are automatically generated.
Click here for the English version.
在体外
陶氏蛋白聚集与药物筛选研究方法
DOI:
10.3791/58570-v
•
09:49 min
•
November 20, 2018
•
Rosa Crespo
,
Wouter Koudstaal
,
Adrian Apetri
1
Janssen Prevention Center
,
Janssen Pharmaceutical Companies of Johnson and Johnson
Chapters
00:04
Title
00:45
Continuous Mode ThT Aggregation Assay on a Multi-Mode Microplate Reader
06:43
Results: In Vitro Assay to Characterize Tau Protein Aggregation
08:50
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本手稿中描述的 tau 聚集分析模拟了
体内
tau 错误折叠和聚集的预期特征。
Tags
Tau Protein
Alzheimer’s Disease
Tauopathies
Aggregation Assay
Drug Screening
Nucleation-dependent Polymerization
Recombinant Tau
Fluorescence
SEC-MALS
Heparin
Thioflavin-T
Microplate Reader
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