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重组腺相关病毒在成年小鼠中的直接注射
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice
Please note that all translations are automatically generated.
Click here for the English version.
重组腺相关病毒在成年小鼠中的直接注射
DOI:
10.3791/58565-v
•
08:17 min
•
February 15, 2019
•
Dongxiao Li*
1
,
Yundu Li*
2
,
Yunyun Tian
,
Zuoshang Xu
,
Yansu Guo
4
1
Department of Neurology
,
Second Hospital of Hebei Medical University
,
2
No.2 Middle School of Shijiazhuang
,
3
Department of Biochemistry and Molecular Pharmacology
,
University of Massachusetts Medical School
,
4
Key Laboratory of Hebei Neurology
Chapters
00:04
Title
00:50
Mouse Preparation and Direct Intrathecal Adeno-associated Virus (AAV) Delivery
03:16
Immunohistochemical Tissue Preparation
04:46
Immunohistochemistry
06:27
Results: Representative AAV Transduction After Direct Intrathecal Delivery
07:52
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个直接鞘内注射技术使用1% 利多因盐酸在病毒溶液中, 以确保有效的腺病毒传递给小动物, 并建立评分系统, 以预测在中央的传导效率神经系统根据利多卡因引起的短暂虚弱程度。
Tags
Direct Intrathecal Injection
Recombinant Adeno-associated Viruses
Adult Mice
Central Nervous System Diseases
ALS
27 Gauge Needle
Hamilton Syringe
Genome Copies
Prone Position
Biosafety Hood
Pelvic Girdle
L5-L6 Intervertebral Space
Midline Of The Spine
30-degree Angle
Intradural Space
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