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Journal
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Immunology and Infection
/
艰难梭菌
(p) pppp 合成酶的纯化及
体外
活性测定
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
A Purification and
In Vitro
Activity Assay for a (p)ppGpp Synthetase from
Clostridium difficile
Please note that all translations are automatically generated.
Click here for the English version.
艰难梭菌
(p) pppp 合成酶的纯化及
体外
活性测定
DOI:
10.3791/58547-v
•
09:53 min
•
November 03, 2018
•
Astha Pokhrel
,
Asia Poudel
,
Erin B. Purcell
1
Department of Chemistry and Biochemistry
,
Old Dominion University
Chapters
00:04
Title
00:44
Protein Purification by Nickel Affinity Chromatography
03:30
Protein Activity Assay by Thin Layer Chromatography
07:30
Results: Thin Layer Chromatography Reveals that
C. difficile
RSH is an Active ppGpp Synthetase
09:01
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了一种纯化带组氨酸标记的热释蛋白酶的方法, 并利用放射性标记底物和产物的薄层色谱法测定了
体外
的酶活性。酶活性测定广泛适用于任何激酶、核苷酸循环酶或磷转移反应, 其机理包括三磷酸核苷酸水解。
Tags
Protein Purification
In Vitro Activity Assay
(p)ppGpp Synthetase
Clostridium Difficile
Phosphotransfer Enzymes
Enzyme Substrate Specificity
Reaction Kinetics
TLC
SDS-PAGE
Coomassie Blue Staining
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