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Journal
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Immunology and Infection
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体外流动下单核细胞亚群的同时研究
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
Please note that all translations are automatically generated.
Click here for the English version.
体外流动下单核细胞亚群的同时研究
DOI:
10.3791/58509-v
•
09:16 min
•
November 26, 2018
•
Patricia Ropraz
,
Beat A. Imhof
,
Thomas Matthes
,
Bernhard Wehrle-Haller
,
Adama Sidibé
1
Hematology service, Centre Médical Universitaire (CMU), Medical faculty
,
University of Geneva
,
2
Department of Pathology and Immunology, Centre Médical Universitaire (CMU), Medical faculty
,
University of Geneva
,
3
Department of Cell Physiology and Metabolism, Centre Médical Universitaire (CMU), Medical faculty
,
University of Geneva
Chapters
00:04
Title
00:54
Human Umbilical Vein Endothelial Cell (HUVEC) Staining and Human Pan Monocyte Isolation
03:20
Fluidic System Preparation
05:04
Time-lapse Imaging of Monocyte Recruitment Under Flow and Image Analysis
06:56
Results: Representative Monocyte Transmigration Analysis
08:25
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个综合协议, 测量单核细胞亚群贩运在流在体外使用特定的表面标记和共聚焦荧光显微镜。该协议可用于探索顺序招募步骤, 以及使用其他特定的表面标记来描述其他白细胞亚型。
Tags
Monocyte Subpopulations
Leukocyte Adhesion
Trans-endothelial Migration
Hematopoietic Cells
HUVEC
CMFDA
Density Gradient Separation
PAN Monocyte Isolation
Transmigration
Adhesion
M199 Medium
BSA
Article
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