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同源小鼠 B 细胞淋巴瘤模型对 CD19 轿车 T 细胞的临床前评价
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells
DOI:

12:16 min

October 16, 2018

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Chapters

  • 00:04Title
  • 00:55Days 1 & 2: Preparation and Transfection of Plat-E Retroviral Packaging Cell Line
  • 02:11Day 3: Preparation of Virus-Containing Supernatant for Transduction and Isolation and In Vitro Activation of Mouse Splenic T Cells
  • 04:27Day 4: Transduction of Mouse T Cells
  • 06:43Day 5: Round 2 of Transduction
  • 08:33In Vitro Validation of CAR T Cell Activity
  • 09:53Results: Pre-Clinical Evaluation of CD19 CAR T Cells
  • 11:32Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个协议, 用于生产和临床前测试小鼠 CD19 汽车 T 细胞的逆转录病毒转导和利用, 作为一种治疗同源 A20 B 细胞淋巴瘤在 BALB/c 鼠有或没有 lymphodepleting预调理。

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