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凝胶神经元全细胞斑块夹的急性脊髓切片的制备
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons
Please note that all translations are automatically generated.
Click here for the English version.
凝胶神经元全细胞斑块夹的急性脊髓切片的制备
DOI:
10.3791/58479-v
•
08:30 min
•
January 18, 2019
•
Mengye Zhu
,
Daying Zhang
,
Sicong Peng
,
Nana Liu
,
Jing Wu
,
Haixia Kuang
,
Tao Liu
3
1
Department of Pain
,
First Affiliated Hospital of Nanchang University
,
2
Department of Pediatrics
,
First Affiliated Hospital of Nanchang University
,
3
Center for Laboratory Medicine
,
First Affiliated Hospital of Nanchang University
Chapters
00:04
Title
00:39
Preparation of Micropipettes and Agar Block
01:30
Spinal Cord Dissection and Slicing
03:46
Whole-cell Patch-clamp Recordings
06:57
Results: Voltage-clamp Recordings in SG Neurons
08:03
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们描述了
由体外
脊髓切片中的明胶 (sg) 神经元制成的全细胞膜片夹记录的基本步骤。该方法可对 sg 神经元的固有膜特性、突触传递和形态表征进行研究。
Tags
Acute Spinal Cord Slices
Whole-cell Patch-clamp Recording
Substantia Gelatinosa Neurons
Sensory Transmission
Nociceptive Regulation
Chronic Pain
Agar Block
Sucrose-based ACSF
Transcardial Perfusion
Spinal Cord Extraction
Lumbosacral Enlargement
Meninges Removal
Pia Arachnoid Membrane
Ventral/dorsal Roots
Transverse Slices
Parasagittal Slices
Vibratome
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