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利用本地绿色凝胶电泳分离菠菜类黄体蛋白配合物, 用时间相关单光子计数表征
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Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting
Please note that all translations are automatically generated.
Click here for the English version.
利用本地绿色凝胶电泳分离菠菜类黄体蛋白配合物, 用时间相关单光子计数表征
DOI:
10.3791/58470-v
•
08:40 min
•
February 14, 2019
•
Eliezer Schwarz
,
Gary Blanchard
1
Department of Plant Biology
,
Michigan State University
,
2
Department of Chemistry
,
Michigan State University
Chapters
00:04
Title
00:50
Isolation of Crude Thylakoid Membranes from Spinach Leaves
02:51
Solubilization of Thylakoid Membranes for Loading onto Native Gels and Separation of Solubilized Thylakoid Proteins by Native Gel Electrophoresis
04:02
Excision of Thylakoid Complex Bands from Native Green Gels and Collection of Room Temperature Steady-State Fluorescence Spectra
05:01
Time-correlated Single Photon Counting of Green Gel Bands and Data Analysis
06:07
Results: Native Green Gel Electrophoresis and Band Characterization
08:14
Conclusion
Summary
Automatic Translation
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在这里, 我们提出了一个协议, 以分离溶解性类囊化复合物的本地绿色凝胶电泳。绿色凝胶带随后通过时间相关单光子计数 (tcspc) 进行了表征, 并提供了数据分析的基本步骤。
Tags
Thylakoid Protein Complexes
Native Green Gel Electrophoresis
Time-correlated Single Photon Counting
Photosynthesis Research
Chlorophyll
Spinach Leaves
TMK Buffer
Methanol Extraction
Centrifugation
Sample Preparation
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