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Journal
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Immunology and Infection
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成人斑马鱼对 DNA 疫苗临床前筛选的免疫研究
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines
Please note that all translations are automatically generated.
Click here for the English version.
成人斑马鱼对 DNA 疫苗临床前筛选的免疫研究
DOI:
10.3791/58453-v
•
05:39 min
•
October 30, 2018
•
Henna Myllymäki*
1
,
Mirja Niskanen*
1
,
Kaisa Oksanen
2
,
Mika Rämet
3,4,5
1
BioMediTech Institute, Faculty of Medicine and Life Sciences
,
University of Tampere
,
2
MedEngine Oy
,
3
Department of Pediatrics
,
Tampere University Hospital
,
4
Department of Children and Adolescents
,
Oulu University Hospital
,
5
PEDEGO Research Unit, Medical Research Center
,
University of Oulu
Chapters
00:04
Title
00:35
Pulling the Microinjection Needles
01:10
Filling the Micropipette Needles
01:49
Setting the Microinjector and Electroporator for Immunization
02:40
Injection of the DNA Vaccine and Electroporation and Visualization of Antigen Expression
04:04
Results:
Danio rerio
Injected with DNA-based Vaccine
05:04
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们描述了一项关于成人斑马鱼 (
斑马斑马
) 免疫接种的协议, 以 DNA 为基础的疫苗, 并证明了成功的疫苗接种事件的验证。该方法适用于各种感染模型的疫苗候选者的临床前筛选。
Tags
Adult Zebrafish
DNA-based Vaccines
Preclinical Screening
Tuberculosis
Cost-effective
Injection
Plasmid
Micro Manipulator
Electroporator
Anesthesia
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