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Biology
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cd4
+
t 细胞的分离及6色流式细胞仪分析外周血中循环 t-滤泡 (ctfh) 细胞亚群
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JoVE Journal
Biology
Isolation of CD4
+
T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
cd4
+
t 细胞的分离及6色流式细胞仪分析外周血中循环 t-滤泡 (ctfh) 细胞亚群
DOI:
10.3791/58431-v
•
07:39 min
•
January 07, 2019
•
Elliot Byford
,
Matthew Carr
,
Lucia Piñon
,
Matthew J. Ahearne
,
Simon D. Wagner
1
Leicester Cancer Research Centre and Ernest and Helen Scott Haematology Research Institute
,
University of Leicester
,
2
MRC Toxicology Unit
,
University of Leicester
Chapters
00:04
Title
00:43
CD4
+
T Cell Peripheral Whole Blood Isolation and Flow Cytometric Analysis
04:45
Data Analysis
06:07
Results: Representative Circulating T-follicular Helper (cTfh) Cell Subset Analyses
06:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了一种从人外周血中分离和
表征 cd4 + t 细胞亚群的方法。采用流式细胞仪对纯化的 cd4
+
t 细胞进行分析, 以确定 t-滤泡辅助细胞亚群的比例。
Tags
CD4+ T-cells
T-follicular Helper (Tfh) Cells
Circulating T-follicular Helper (cTfh) Cells
Flow Cytometry
Biomarker
Autoimmune Disease
B-cell Non-Hodgkin’s Lymphoma
Peripheral Blood
Cell Isolation
Cell Subsets
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