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体外3D 球形培养模型小鼠前列腺叶的鉴定、组织学特征及解剖
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Cancer Research
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JoVE Journal Cancer Research
Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models
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体外3D 球形培养模型小鼠前列腺叶的鉴定、组织学特征及解剖

DOI:
10.3791/58397-v

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08:43 min

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September 18, 2018

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, , ,
1Department of Urology,SUNY Upstate Medical University, 2Department of Biochemistry and Molecular Biology,SUNY Upstate Medical University, 3Laboratory of Comparative Pathology,Memorial Sloan-Kettering Cancer Center, 4Boulder BioPATH, Inc

Chapters

  • 00:04TItle
  • 00:35Urogenital System (UGS) Dissection
  • 02:04Prostate Gross Anatomy and Individual Lobe Microdissection
  • 02:51Tissue Processing for 3D Culture
  • 04:41Cell Plating and Culture and Sphere Harvest
  • 06:38Results: Representative Mouse Prostate Lobe Analyses
  • 07:41Conclusion

Summary

Automatic Translation

Automatic Translation

基因工程鼠是研究前列腺癌机制的有用模型。在这里, 我们提出了一个协议, 以识别和解剖小鼠泌尿生殖系统前列腺裂片, 区分他们的组织学, 并分离和培养的原发性前列腺细胞体外作为球体的下游分析。

Tags

ProstateMouse ModelLobesSpheroid CultureIn Vitro AnalysisGenetically-engineeredProstate CancerUrogenital SystemFat RemovalSeminal VesiclesDorsal LobesLateral LobesVentral LobesAnterior LobesDMEM

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