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磷流式细胞仪与荧光细胞条形码用于单细胞信号分析和生物标志物发现
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Cancer Research
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JoVE Journal Cancer Research
Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery
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磷流式细胞仪与荧光细胞条形码用于单细胞信号分析和生物标志物发现

DOI:
10.3791/58386-v

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08:38 min

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October 04, 2018

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1Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership,University of Oslo, 2K. G. Jebsen Centre for B cell malignancies and K. G. Jebsen Centre for Cancer Immunotherapy,University of Oslo

Chapters

  • 00:04Title
  • 00:32Preparation, Stimulation, and Fixation of Cells
  • 01:50Fluorescent Cell Barcoding
  • 03:30Cell Permeabilization and Intracellular Antigen Staining
  • 06:12Results: Phospho Flow Cytometry Analysis with Fluorescent Cell Barcoding
  • 08:02Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 提出了一种中-高通量分析在细胞级蛋白质磷酸化事件的协议。磷流式细胞术是表征信号畸变、识别和验证生物标志物、评估药效学的有力手段。

Tags

Phospho Flow CytometryFluorescent Cell BarcodingSingle Cell Signaling AnalysisBiomarker DiscoveryCell BiologyImmunologySignaling PathwaysStimuliDrugsHigh ThroughputRPMI 16/40 MediumFixed BufferAnti-IgMPBSBarcoding ReagentsPerm BufferCompensation Control

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