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Cancer Research
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用水凝胶微室阵列 (HMCA) 板进行肿瘤细胞侵袭和抗转移药物筛选的分析
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Cancer Research
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JoVE Journal
Cancer Research
Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array (HMCA)-based Plates
Please note that all translations are automatically generated.
Click here for the English version.
用水凝胶微室阵列 (HMCA) 板进行肿瘤细胞侵袭和抗转移药物筛选的分析
DOI:
10.3791/58359-v
•
08:32 min
•
October 25, 2018
•
Orit Ravid-Hermesh
,
Naomi Zurgil
,
Yana Shafran
,
Elena Afrimzon
,
Maria Sobolev
,
Yaron Hakuk
,
Zehavit Bar-On Eizig
,
Mordechai Deutsch
1
Physics Department
,
Bar-Ilan University
Chapters
00:04
Title
01:17
Hyrdogel Micro-chamber Array (HMCA) Plate Embossing
02:28
Cell Loading and Spheroid Formation
04:26
Invasion Assay Acquisition and Analysis
06:06
Results: Representative Influence of Extracellular Matrix (ECM) Composition on Spheroid Invasion
07:25
Conclusion
Summary
Automatic Translation
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Automatic Translation
针对入侵检测的性能, 提出了一种基于 HMCA 的成像板。该板块有助于形成三维 (3D) 肿瘤旋转椭球体和测量癌细胞侵入细胞外基质 (ECM)。通过半自动分析实现了入侵检测的定量化。
Tags
3D Cell Culture
Hydrogel Micro-chamber Array
Tumor Spheroid
Anti-metastatic Drug Screening
Cell Invasion
PDMS Stamp
Agarose Gel
UV Sterilization
Cell Seeding
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