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Cancer Research
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3维细胞培养系统研究膀胱癌侵袭和评价治疗方法
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Cancer Research
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JoVE Journal
Cancer Research
3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
Please note that all translations are automatically generated.
Click here for the English version.
3维细胞培养系统研究膀胱癌侵袭和评价治疗方法
DOI:
10.3791/58345-v
•
09:24 min
•
September 13, 2018
•
Yin Wang
,
Mark L. Day
,
Diane M. Simeone
,
Phillip L. Palmbos
1
Departments of Internal Medicine, Hematology/Oncology Division, Rogel Cancer Center
,
University of Michigan Medical Center
,
2
Department of Urology, Division of GU Oncology, Rogel Cancer Center
,
University of Michigan Medical Center
,
3
Departments of Surgery and Pathology, Perlmutter Cancer Center
,
NYU Langone Health
Chapters
00:04
Title
01:03
Growing Cancer Spheroids
02:24
Preparing the 3D Culture Chamber
03:50
Live Cell Time-lapse Imaging
04:24
Preparation of Sample Block Containing Cancer Spheroids for Frozen Tissue Sectioning
05:36
Immunofluorescence Imaging for Frozen Sectioned Cancer Spheroids
07:18
Results: Invasive Bladder Cancer Tumor Spheroids Formed from Cell Lines and Primary Tumors
08:45
Conclusion
Summary
Automatic Translation
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Automatic Translation
控制膀胱癌的过程代表了生物标志物和治疗发展的机会。在这里, 我们提出了一个膀胱癌入侵模型, 其中包括3维的肿瘤球体文化, 时间推移成像和共焦显微术。该技术可用于定义侵入过程的特征和筛选治疗药物。
Tags
3D Cell Culture
Bladder Cancer
Invasion
Therapeutics
Tumor Spheroids
Collagen
Primary Tumors
Cell Lines
Microscopy
Immunostaining
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