Journal
/
/
3维细胞培养系统研究膀胱癌侵袭和评价治疗方法
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
DOI:

09:24 min

September 13, 2018

, , ,

Chapters

  • 00:04Title
  • 01:03Growing Cancer Spheroids
  • 02:24Preparing the 3D Culture Chamber
  • 03:50Live Cell Time-lapse Imaging
  • 04:24Preparation of Sample Block Containing Cancer Spheroids for Frozen Tissue Sectioning
  • 05:36Immunofluorescence Imaging for Frozen Sectioned Cancer Spheroids
  • 07:18Results: Invasive Bladder Cancer Tumor Spheroids Formed from Cell Lines and Primary Tumors
  • 08:45Conclusion

Summary

Automatic Translation

控制膀胱癌的过程代表了生物标志物和治疗发展的机会。在这里, 我们提出了一个膀胱癌入侵模型, 其中包括3维的肿瘤球体文化, 时间推移成像和共焦显微术。该技术可用于定义侵入过程的特征和筛选治疗药物。

Related Videos

Read Article