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体内
新生小鼠皮层神经元的双光子成像
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
In Vivo
Two-photon Imaging of Cortical Neurons in Neonatal Mice
Please note that all translations are automatically generated.
Click here for the English version.
体内
新生小鼠皮层神经元的双光子成像
DOI:
10.3791/58340-v
•
06:24 min
•
October 18, 2018
•
Hidenobu Mizuno
2,3
,
Shingo Nakazawa
3
,
Takuji Iwasato
3
1
International Research Center for Medical Sciences (IRCMS)
,
Kumamoto University
,
2
Division of Neurogenetics
,
National Institute of Genetics
,
3
Department of Genetics
,
SOKENDAI (The Graduate University for Advanced Studies)
Chapters
00:04
Title
00:33
Exposing the Skull
01:29
Cranial Window Preparation
03:29
Two-Photon Imaging
04:59
Results:
In vivo
Two-Photon Imaging of Cortical Neurons in Neonatal Mice
05:57
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了一种用于成像新生小鼠大脑皮层的
活体
双光子成像协议。该方法适用于分析皮层神经元的发育动态、控制神经元动力学的分子机制以及疾病模型神经元动力学的变化。
Tags
In Vivo
Two-photon Imaging
Cortical Neurons
Neonatal Mice
Neuroscience
Neuron Morphology
Cranial Window
Anesthesia
Craniotomy
Agarose Gel
Cover Slip
Dental Cement
Titanium Bar
Analgesic
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