/
/
Fabricage van anisotrope polymere kunstmatige antigeen presentatie van cellen voor CD8 + T cel activatie
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Biomedical Engineering, Translational Tissue Engineering Center, Institute for Nanobiotechnology,Johns Hopkins University School of Medicine, 2Biomedical Engineering, Translational Tissue Engineering Center, Institute for Nanobiotechnology, Ophthalmology, Oncology, Neurosurgery, Materials Science and Engineering, Chemical and Biomolecular Engineering, and the Bloomberg-Kimmel Institute for Cancer Immunotherapy,Johns Hopkins University School of Medicine
Chapters
- 00:04Title
- 00:49Tunable Spherical Poly(Lactic-Co-Glycolic Acid) (PLGA) Fabrication
- 03:30Tunable Polymeric Particle Fabrication
- 06:21Surface Protein Conjugation for Artificial Antigen Presenting Cell (aAPC) Creation
- 08:01Results: Representative Spherical and Ellipsoidal Particle Characterization
- 09:49Conclusion
Hier presenteren we een protocol voor het snel en reproducibly genereren van biologisch geïnspireerde, biologisch afbreekbare articifical antigeen presentatie van cellen (aAPC) met afstembare grootte, vorm en oppervlakte-proteïne presentatie voor T cel expansie ex vivo of in vivo .
